Journal: Cell communication and signaling : CCS

Article Title: O-GlcNAcylated FTO promotes m6A modification of SOX4 to enhance MDS/AML cell proliferation.

doi: 10.1186/s12964-025-02058-6

Figure Lengend Snippet: Fig. 2 O‑GlcNAcylation weakens FTO expression. (A) Volcano diagram representing FTO expression after OSMI-1 treatment in KG1a cells; p-val ue < 0.05,|Fold change| > 1.5. (B) The correlation between O-GlcNAc levels and the survival of AML patients was explored using upper quartile analysis through the Kaplan-Meier Plotter. (C) The association between O-GlcNAc and FTO in a cohort of 14 MDS/AML patients by ELISA. (D) Western blot detec tion of FTO level in cKit + cells of the bone marrow from MLL-AF9-induced leukemia mice. (E) FTO level in primary CD34 + cells from MDS/AML patients after 20 µM DMSO and 20 µg/mL OSMI-1 treatment. (F-G) FTO level in KG1a and SKM1 cells after TMG and OSMI-1 treatment

Article Snippet: CD34+ cells were treated with 20 μM DMSO and 20 μg/mL OSMI-1 (MCE, NJ, USA) for 48 h, respectively.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Journal of Cancer

Article Title: Talin1 Mediates Tumor-Nerve Interactions in Prostate and Breast Cancer Cells

doi: 10.7150/jca.127292

Figure Lengend Snippet: Human Neural Progenitor Cells (NPCs) display increased neurite outgrowth when cultured with exosomes and CM from MCF-7 Snail cells compared to MCF-7 Neo. MCF-7 Neo or MCF-7 Snail was cultured in exosome-depleted medium (Exo-D medium) for 3 days, and conditioned medium (CM) was collected, or exosomes were isolated from the CM. These were subsequently cultured with NPCs for 2 days. (A) Images of NPC after incubation in NPC regular medium, Exo-D medium, exosomes or CM with or without Talin1 inhibitor mH4 were captured with a Nikon Eclipse TE2000-S Inverted Fluorescence Microscope. (B) The percentage of NPCs with neurite outgrowth and average neuron length was measured utilizing Image J Fiji Just software in NPCs cultured with NPC medium, Exo-D medium, and Exo-D medium plus NGF-beta as a positive control. The percentage of NPCs with neurite outgrowth and average neuron length of NPCs cultured with (C) exosomes isolated from MCF7-Neo and MCF7-Snail CM or (D) CM from MCF7-Neo and MCF7-Snail was analyzed using ImageJ Fiji Just software. Statistical analyses were done using GraphPad Prism software (one-way ANOVA, Šídák's multiple comparisons test, ****p< 0.0001, ***p< 0.001, **p < 0.01, *p < 0.05). Bars represent the SD of the mean. Results are representative of 3 experiments performed independently.

Article Snippet: Neural Progenitor Cells (ACS-5004), NPC Growth Medium (ATCC ACS-3003), DMEM: F12 (ATCC 30-2006) and Cell Basement Membrane (ACS-3035) were purchased from American Tissue Cell Culture (ATCC), Manassas, VA. Talin1 inhibitor, mH4 and F3 protease inhibitor control, was obtained from Dr. Kensei Tsuzaka, Kaytee Bio, Co. & Ltd., Chiba, Japan. mH4 was dissolved in DMSO to a stock solution of 14 mg/ml.

Techniques: Cell Culture, Isolation, Incubation, Fluorescence, Microscopy, Software, Positive Control

Journal: Journal of Cancer

Article Title: Talin1 Mediates Tumor-Nerve Interactions in Prostate and Breast Cancer Cells

doi: 10.7150/jca.127292

Figure Lengend Snippet: Human neural progenitor cells (NPCs) display reduced neurite outgrowth with exosomes and CM from C4-2 Snail knockdown (C4-2 E8) cells compared to C4-2 Non-silencing (NS) cells. C4-2 NS or C4-2 E8 was cultured in exosome-depleted medium (Exo-D medium) for 3 days, and conditioned medium (CM) was collected, or exosomes were isolated from the CM. These were subsequently cultured with NPCs for 2 days. (A) Images of NPC after incubation in NPC regular medium, Exo-D medium, exosomes, or CM with or without Talin1 inhibitor mH4 were captured with a Nikon Eclipse TE2000-S Inverted Fluorescence Microscope. The percentage of NPCs with neurite outgrowth and average neuron length of NPCs cultured with (B) exosomes isolated from MCF7-Neo and MCF7-Snail CM or (C) CM from MCF7-Neo and MCF7-Snail was analyzed using ImageJ Fiji Just software. Statistical analyses were done using GraphPad Prism software (one-way ANOVA, Šídák's multiple comparisons test, ****p< 0.0001, **p < 0.01, *p < 0.05). Bars represent the SD of the mean. Results are representative of 3 experiments performed independently.

Article Snippet: Neural Progenitor Cells (ACS-5004), NPC Growth Medium (ATCC ACS-3003), DMEM: F12 (ATCC 30-2006) and Cell Basement Membrane (ACS-3035) were purchased from American Tissue Cell Culture (ATCC), Manassas, VA. Talin1 inhibitor, mH4 and F3 protease inhibitor control, was obtained from Dr. Kensei Tsuzaka, Kaytee Bio, Co. & Ltd., Chiba, Japan. mH4 was dissolved in DMSO to a stock solution of 14 mg/ml.

Techniques: Knockdown, Cell Culture, Isolation, Incubation, Fluorescence, Microscopy, Software